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Frequently Asked Questions

What is Dharmacon Edit-R CRISPR-Cas9?

The Dharmacon Edit-R CRISPR-Cas9 platform greatly simplifies the workflow of permanently knocking out genes. Our approach includes predesigned, ready-to-use DNA and RNA components and enables fast assessment of multiple target sites per gene for multiple genes.

Why choose Gege Dharmacon RNA synthesis?

GE Dharmacon RNA synthesis chemistry provides researchers with high-quality custom single-stranded RNA, siRNA, synthetic sgRNA, and microRNA with an extensive range of modifications, synthesis scales, and purification choices. Also available are DNA only oligos, as well as RNA:DNA hybrids.

Which Edit-R components can be co-transfected with dharmafect duo?

Recommended guidelines for co-transfection of the Edit-R components (Cas9 Nuclease expr plasmid, synthetic tracrRNA and crRNA) using DharmaFECT Duo.

What is a dharmafect crRNA?

a custom crRNA designed to the target site of interest. All three components are co-transfected into the mammalian cell of choice using the Dharmacon DharmaFECT Duo Transfection Reagent to perform gene knockout. What does this mean for you?

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