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Frequently Asked Questions

What is Dharmacon Edit-R CRISPR-Cas9?

The Dharmacon Edit-R CRISPR-Cas9 platform greatly simplifies the workflow of permanently knocking out genes. Our approach includes predesigned, ready-to-use DNA and RNA components and enables fast assessment of multiple target sites per gene for multiple genes.

Why choose Dharmacon for siRNA?

Broadly useful across many applications and in any cell line, siRNA offers a straightforward and widely cited technique for functional gene silencing. For over 20 years, Dharmacon has remained the most trusted name in high quality siRNA design and synthesis. Select a predesigned siRNA product line or design your own siRNA with custom modifications.

How do the Cas9 protein and tracrRna interact with CRISPR-Cas9?

The Cas9 protein and tracrRNA are designed to interact and work with any crRNA generated by the CRISPR Design Tool. The Edit-R CRISPR-Cas9 system includes the three critical components required for gene editing in mammalian cells, based on the natural S. pyogenes system:

Which Edit-R components can be co-transfected with dharmafect duo?

Recommended guidelines for co-transfection of the Edit-R components (Cas9 Nuclease expr plasmid, synthetic tracrRNA and crRNA) using DharmaFECT Duo.

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